ObaCell® SVF Fat-on-a-Chip Kit
ObaCell® was created as a tool for disease modeling and drug screening; moreover, it's a versatile plug-and-play system capable of seamless integration with other organ-on-a-chip models, offering boundless potential for transformative research and innovation.
ObaCell® SVF Fat-on-a-Chip Kit includes all of the components you need to create a functional adipose organoid model in your lab in ONLY 3 weeks! Customize your kit by choosing a unique cell donor from our diverse inventory that best represents the prevalent population modeled in your study.
The Stromal Vascular Fraction (SVF) represents a native multicellular population extracted from adipose tissue including endothelial progenitor cells, mesenchymal stem cells, and immune cells.
Endpoint analyses capable of this model include, but are not limited to, brightfield and fluorescent imaging, confocal microscopy, high-throughput microscopy, cell viability staining, metabolic activity assays via alamarblue or similar reagents, cell retrieval, histological staining, secretome analysis, cytokine profiles, and RNA and protein expression.
Key Features
- Multi-cellular population retained within hydrogel scaffold
- In-vitro self-assembled organoids
- Physiologically and functionally relevant human-derived adipose model
- Matrix protein profile supports cell growth, motility, and vascular response
Kit Components
Stromal Vascular Fraction Cells (5 million cells)
ObaGel® Human-derived Hydrogel (5 ml)
ObaVate Activation Agent (15 ml)
StromaQual3D Maintenance Media (30 ml)
AdipoQual3D Adipogenic Differentiation Media (30 ml)
ObaZolve Cell Retrieval Agent (30 ml)
- Kit includes reagents suited for 96 test wells in a 96-well plate format. Please view our protocols here to see an optimized plate layout.
Price $6,845
SKU OS-306-01
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Relevant Publications
2024
Adipose Tissue in Breast Cancer Microphysioloigical Models to Capture Human Diversity in Preclinical Models. Int. J. Mol. Sci. 2024, 25, 2728.
2021
Adipose-derived cells: building blocks of three-dimensional microphysiological systems. Biomater Transl. 2021 Dec 28;2(4):301-306.
Human Adipose-Derived Stromal/Stem Cell Culture and Analysis Methods for Adipose Tissue Modeling In Vitro: A Systematic Review. Cells. 2021 Jun 3;10(6):1378.
2020
Human Adipose Derived Cells in Two- And Three-Dimensional Cultures: Functional Validation of an In Vitro Fat Construct. Stem Cells Int. 2020 Jun 10
Fat-on-a-Chip Models for Research and Discovery in Obesity and Its Metabolic Co-Morbidities Tissue Eng Part B Rev. 2020 Mar 26.
Adipose Tissue-Derived Stem Cells Retain Their Adipocyte Differentiation Potential in Three-Dimensional Hydrogels and Bioreactors. Biomolecules. 2020 Jul; 10(7): 1070.
Safety of Human Adipose Stromal Vascular Fraction Cells Isolated With a Closed System Device in an Immunocompetent Murine Pressure Ulcer Model. Stem Cells Dev. 2020 Apr 1
Arguments for a Different Regulatory Categorization and Framework for Stromal Vascular Fraction. Stem Cells and Development, January 6, 2020
2017
Adipose Stromal Vascular Fraction-Mediated Improvements at Late-Stage Disease in a Murine Model of Multiple Sclerosis. Stem Cells vol. 35 pg. 532-544